1.Qiagen公司ffpe 样本rna抽提试剂盒
货号80234
AllPrep DNA/RNA FFPE Kit (50)
50 RNeasy MinElute Spin Columns, 50 QIAamp MinElute Spin Columns, Collection Tubes, RNase-Free Reagents, and Buffers
从福尔马林固定、石蜡包埋的组织切片中同时纯化基因组DNA和总RNA（包括小RNAs）

• 使用小样本量即可获得大量纯化产物
• 在纯化同时，保持DNA和RNA的完整性
• 高效分离RNA和DNA
• 对同一FFPE样本进行全面的DNA和RNA分析

如要对基因组学和转录组学数据进行可靠比较，则需从同一样本中纯化DNA和RNA。AllPrep DNA/RNA FFPE Kit使用先进的溶解方法，从福尔马林固定、石蜡包埋的组织样本中纯化DNA和RNA。纯化产物适用于real-time PCR和焦磷酸测序等应用。
从FFPE样本中纯化DNA和RNA。
[A] 从多个大鼠FFPE组织样本中纯化基因组DNA并储存于室温。使用AllPrep DNA/RNA FFPE Kit或QIAamp DNA FFPE Tissue Kit（专用于从FFPE样本中纯化DNA的试剂盒，作为对照）进行纯化，两种试剂盒都含有RNase消化试剂。采用吸光度测定方法检测每个20 μm组织切片的DNA产量。[B] 从多个大鼠FFPE组织样本中纯化RNA并储存于室温。使用AllPrep DNA/RNA FFPE Kit或RNeasy FFPE Tissue Kit（专用于从FFPE样本中纯化DNA的试剂盒，作为对照）进行纯化。采用吸光度测定方法检测每个10 μm组织切片的RNA产量。从FFPE组织中纯化DNA或RNA时，AllPrep Kit的表现与专用的纯化试剂盒表现相当。

2. Invitrogen™RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE  货号：  AM1975  规格40 preps​​​​​​​

 

描述

The RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE is for the extraction of total nucleic acid from formalin- or paraformalin-fixed, paraffin-embedded (FFPE) tissues. Sufficient reagents are included for 40 purifications from up to four 20 µm sections, or up to 35 mg of unsectioned, core samples each. Features of the RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE:

• Optimized for isolation of total nucleic acids, including microRNAs, from FFPE tissue
• No overnight Proteinase K digestion required—deparaffinize in the morning and perform qRT-PCR in the afternoon
• Typical yields are >50% of those of unfixed tissue
• Recovered nucleic acids are suitable for next generation sequencing, real-time RT-PCR, PCR, mutation screening, and microarray analyses